Safe use of laboratory liquid nitrogen

  Liquid nitrogen needs to be used frequently in laboratory research, such as high pressure freezing, cryosection, and cryo-electron microscopy, but the temperature of liquid nitrogen is extremely low, so the safe use of liquid nitrogen is also very important.

  One: application of liquid nitrogen

  1: frozen sample

  2: as a second refrigerant when it is put into freezing

  3: Freezing instrument

  4: Store samples

  2. Physical properties of liquid nitrogen

  Melting point 63K/-210°C

  Boiling point 77K/-196"C

  Face-to-face: 1: Frostbite (in the laboratory, the boiling temperature is generally used, and the liquid hydrogen has a Leidenfrost Effect effect. Temporary protection by the insulating gas layer)

  How to prevent: Do not directly control with liquid nitrogen. Do not take a long time to contact the body of the nitrogen body, do not touch the liquid oxygen for a long time, which phase is wet clothes.

  2: Insufficient oxygen (1L of liquid nitrogen can be vaporized into 700L of nitrogen to reduce the oxygen content in the air. Oxygen in the atmosphere

  The most 21%, less than 18% will damage the health of the body and mind, less than 11% will have great risks, such as injury or even death. example

  For example, a closed 3*3*2.5 space, 10L of liquid nitrogen can reduce the oxygen content to 16%)

  How to prevent: Do not use a lot of liquid hydrogen in a small space, work in a well ventilated environment, use an oxygen monitor and calibrate frequently, do not work alone.

  3: Explosion (in a confined space, vaporized liquid nitrogen will quickly increase the pressure of the container. For example: filled in a sealed container

  50% of the liquid nitrogen pressure can reach 350bar, if the container can not withstand such a large pressure, it will explode quickly. )

  How to prevent: Just cover the liquid nitrogen with a less sealed lid and use a professional liquid nitrogen tank.

  4: Condensation of liquid oxygen (oxygen in the air will condense in liquid hydrogen, and liquid oxygen will ignite or react with organic materials.)

  How to prevent: Do not store liquid nitrogen in the container for too long.


 

  Three: Summary

  Understand the risks of liquid nitrogen, understand the regulations of the laboratory and comply with them.

  If you are interested in the liquid nitrogen containers, storage tanks, drums & containers or need to consult, please click on our online customer service.Welcome sending your inquiry.

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