Liquid nitrogen bucket structure: Let each embryo baby have its own "home"

With the development of artificial assisted reproductive technology, more and more couples who cannot naturally conceive have become the wish of Baoma and Baoda. But do you know? At present, embryo freezing is one of the mature methods of preserving fertility. In recent years, the clinical application of frozen embryos has been increasing year by year. Frozen embryos are stored in a liquid nitrogen environment at minus 196 ° C, so that the embryos are preserved for a long time.

US frozen transplantation showed a growing trend: in 2007, frozen transplantation accounted for 18% of the total transplant cycle, and in 2015 it increased to 41%; European frozen transplant showed a growth trend: in 2007, frozen transplant accounted for 20% of the total transplant cycle, and in 2014 it increased to 38%. %; China's whole embryo freezing rate also showed an increasing trend: frozen transplants accounted for 29.5% of the total transplant cycle in 2013, and increased to 53.4% ??in 2017.


As mentioned above, frozen embryos are becoming more widely used and the rate of frozen embryo transfer is increasing. Why do you want the embryo baby to have a home in the liquid nitrogen tank? Originally, in the actual work, there are 5-6 buckets in a large liquid nitrogen tank, 20-40 in each bucket. A frozen scaffold with 4-5 frozen rods on each scaffold and 1-2 embryonic babies on each freezing rod. That is to say, in a bucket, there are 160-400 embryo babies, and a liquid nitrogen tank can have 800-2400 embryo babies. It should be noted that although the embryos on each of the freezing rods are the same couple, different husbands are on different freezing rods and on different brackets. When freezing and thawing embryos, the embryologist needs to quickly and accurately place between the liquid nitrogen and the normal temperature after the repeated examinations. Before the embryo transfer operation, the staff needs to quickly and accurately find the designated embryo baby of the couple who need to be transplanted. The process of finding the designated number of embryos is based on the length of time in the low temperature environment of liquid nitrogen. Although it does not affect the success rate of transplantation, it affects the work efficiency. In the previous liquid nitrogen bucket, the frozen stent was immersed in liquid nitrogen liquid. Under the action of liquid mechanics, the numbered frozen stent (about 20-40) will become disordered after being placed in liquid nitrogen, and needs to be thawed. When the embryo is born, it is a test of the "eyesight" of the experimenter. With the increase in the freeze-thaw cycle, the workload of laboratory staff is increasing.


To this end, Liu Minghui and Li Yuan, Department of Reproductive Medicine, Beijing Chaoyang Hospital, designed a liquid nitrogen bucket containing a “skeleton”. When frozen, the embryologist can place the embryonic scaffold in an orderly manner according to the skeleton number. When thawed, the embryonic baby's home can be quickly found according to the ordered skeleton number. In this way, just like letting each embryo baby live in a new "home" of unified planning, it is a quick and easy thing to quickly find the designated embryo baby.


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